5-Nitroindole

5-Nitroindole is a hydrophobic aromatic compound, and can be used as a universal base analog in oligonucleotides. Incorporation of universal bases into oligos is desirable in cases when either imprecise or random base-pairing is required, and the resulting “mis-matched” complements need to be stable. 5-Nitroindole does not discriminate between the four natural nucleotide bases in duplex formation. Substitution of this chemical as a universal base is less destabilizing towards the ends of oligos than towards the center. Substitutions at, or within eight bases of, the 3’-end produce primers that generally are ineffective at priming.

Product Details:

• PubChem CID : 22523
• Chemical Names : 5-Nitroindole; 6146-52-7; 5-Nitro-1H-indole; 1H-Indole, 5-nitro-; INDOLE, 5-NITRO-; UNII-O2BHX6EDBN
• Molecular Formula : C₈H₆N₂O₂
• Molecular Weight : 162.148 g/mol
• IUPAC Name : 5-nitro-1~{H}-indole
• CAS No : 6146-52-7
• EC Number : 228-153-0
• Monoisotopic Mass : 162.043 g/mol
• Exact Mass : 162.043 g/mol